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Selective Media for Organisms

 

Organism

Medium

Pseudomonas

Cetrimide agar

Gonorrhoea

Thayer Martin and Chacko Nair egg enriched medium.

Bordetella Pertussis Bordet- Genghou medium
Brucella Castaneda agar
T.B. Bacillus Lowenstein- Jensen’s Medium.
Borrelia Noguchi’s medium
Corynebacterium Loeffler’s medium
Staphylococci 8- 10 % Nacl Tellurite medium in salt broth
Shigella Deoxycholate- Citrate agar
Clostridia Robertson’s cooked meat broth, Rabbitserum ( Fletcher Stuarts Medium)
Leptospirosis Chorio- Allantoic membrane of Chick embryo
Anti body sensitivity test 1. Nutrient agar 2. Muller Hinton agar
Transport of Streptococci Pikes medium
Transport of cholera organisms a) Venkatraman- Ramakrishnan Medium b)Cary- Blair Medium
Plating of Cholera organisms a) Alkaline Bile salt agar b) G.T.T.A medium c) T.C.B.S medium
Salmonella Bile broth , Wilson Blair Medium, Selenite – F and Tetrathionate
Anthrax Polymixin, Lysozome, EDTA, Thallous Acetate ( PLET)
Meningococci Muller Hinton Agar
Candida Sabouraud’s medium

 

Dark ground Illumination helps to detect

1. Leptospirosis 2. Treponemes 3. Borrelia

All cocci are Gram positive except Neisseria

All Bacilli are Gram Negative except D- Diptheria, A- Actinomycosis, T- Tetani Cl, T- Tuberculosis Myco, A- Anthrax (DATTA)

 

Generation time of:

Lepra Bacillus

20 days roughly

Tuberculosis Bacillus

20 Hours

Coliforms

20 minutes

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